PROTEIN-PROTEIN INTERACTION AND COARSE GRAINED SIMULATION STUDY OF GLIOBLASTOMA MULTIFORME REVEALS NOVEL PATHWAYS OF GPR17

Abstract

Studies of receptor mediated signaling networks in neuronal cells provide a unique opportunity to uncover the basis of many diseases. Receptor signaling cascades proceed from the cell surface, where extra cellular factors interact with their specific receptors e.g. G Protein Coupled Receptors (GPR). Recent studies have shown that the activation or suppression of GPR17 in diseased neuronal cells has potential impact in altering the tumor conditions. We identified many hundred times expressions of GPR17 in Glioblastoma Multiforme (GBM) from the RNA-Seq data. We also observed many other genes having similar expression patterns with GPR17, indicating possible connections of this receptor with diverse gene products. We performed a coarse-grained simulation of ∼ 500 proteins inside a cytoplasm like a box with solvent water molecules. The summarized protein interaction networks resulted from a coarsegrained simulation and large scale protein-protein docking reveals novel molecular connections and pathways.